FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740120
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC249913.376614603037345No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC189812.564584121493812No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC181502.4523050316164947No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC140381.8967194508998542No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA111101.501107928444036No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG54470.7359617359347133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54010.7297465275901205No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA13170.1779441171701886No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG12030.16254120953358914No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10480.14159865967680918No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA10280.13889639517916014No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9170.12389882721720802No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT8860.11971031724585203No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA8410.1136302221261417No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8120.10971193860455063No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7890.10660433443225423No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG7690.10390206993460521No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA7450.10065935253742637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG54150.0129.967771
GTCTCTC71150.0123.187512
AGTCTCT73350.0119.836161
TCAGCTG73850.0119.416717
AGCTGGT74700.0117.9535459
TCTCTCA74950.0117.36713
CAGCTGG75950.0115.822688
CTCAGCT76400.0115.375696
CTCTCAG77650.0113.062314
TCTCAGC81150.0108.452185
TACGGCG301.6533178E-596.020726
ATACGGC353.5516936E-582.3090445
TTCCGAT554.5996785E-878.567725
GGCTCAA91550.076.8273247
CTCAAAC91700.076.696479
GCTCAAA92500.076.033158
CTGATGG94400.074.751292
GATGGCT94200.074.390514
ATGGCTC97100.072.391275
TGGCTCA97200.072.2378166
Produced by FastQC (version 0.11.5)