FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599279
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAG1050.0130.324683
CGATTGA2400.0126.058721
CCGGTAG1100.0124.42161
TAGTTGG2550.0118.643511
GGTATTG2700.0117.388031
CGACGGA800.0116.995939
AGCGTAT1900.0113.7372061
ACCGCGA900.0112.03352
TACGGTA2200.0111.3245851
GGCGTAC650.0110.765379
CGAACAT2950.0109.88171
ATTACGT1050.0109.74713
ATTGAAG1050.0109.74713
TAGCCGG1550.0106.888511
GCGTTGC950.0106.1015859
ACGCCGA1700.0105.931711
TTAACTA750.0105.631592
AACCGCG2050.0105.414971
TAAGTGC2050.0105.414971
CGGTATC2050.0105.397373
Produced by FastQC (version 0.11.5)