FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599279
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC216543.613342032675932No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC164582.7463001373316938No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC156802.6164774670896196No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC120682.0137531934207606No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA102221.7057163691702861No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG55810.9312857617236712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31430.5244635637157318No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA12170.20307736463316753No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA9060.15118166997341806No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG8970.14967986530480795No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG8760.14617565441138433No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT8060.1344949514333057No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG8000.13349374832089894No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7910.13199194365228883No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA7360.12281424845522702No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG7180.11981063911800681No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA7100.11847570163479781No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA6330.1056269283589113No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC6200.10345765494869669No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT6020.10045404561147646No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT6000.1001203112406742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG44200.0129.92961
AGTCTCT60100.0123.801531
GTCTCTC60050.0123.368142
TCAGCTG62000.0119.258717
TCTCTCA62350.0118.7180253
AGCTGGT62800.0117.510219
CAGCTGG63450.0116.760278
CTCAGCT65850.0112.614116
CTCTCAG65950.0112.081494
TCTCAGC68700.0107.418355
ATACGGC259.648338E-486.39585
CTTCCGA353.5452336E-582.336674
TTCCGAT353.5569952E-582.2817155
GGCTCAA76900.075.179697
CTCAAAC77400.074.6940469
GCTCAAA78000.074.211788
CTGATGG78850.073.186992
GATGGCT79300.072.8622364
CCGATCT406.894994E-571.99657
ATGGCTC81700.070.85095
Produced by FastQC (version 0.11.5)