FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005282018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583747
Sequences flagged as poor quality0
Sequence length8
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATATTC57399498.32924194899503No Hit
TGATATTA26170.4483106551297052No Hit
TGATAATC11720.2007719097485726No Hit
AGATATTC11080.18980825597390652RNA PCR Primer, Index 37 (100% over 8bp)
TGAAATTC10330.1769602242067197No Hit
TGATATAC8040.1377309005442426No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)