FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583747
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC212763.644729651715555No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC161192.7612989874037894No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC155812.669135772860503No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC118492.029817712125287No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA97441.6692162871929106No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG52180.8938803968157438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30720.5262553811839719No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA11310.19374831904917714No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG9840.168566176785491No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT8130.13927266435630503No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG8060.13807351472470095No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA7390.12659593967934737No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG7240.12402633332591004No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7140.12231326242361845No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA7000.11991496316041024No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC6910.11837319934834784No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA6880.11785927807766035No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG6530.11186352991963985No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA6220.10655301012253596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG43550.0130.840131
GTCTCTC58550.0121.9658662
AGTCTCT58900.0121.632191
TCAGCTG59700.0120.35587
AGCTGGT61050.0118.048169
TCTCTCA61500.0116.963433
CAGCTGG63200.0114.374038
CTCTCAG63100.0113.730184
CTCAGCT63800.0113.0824056
TCTCAGC66550.0107.887095
CCGATCT703.274181E-1182.28167
GGCTCAA75950.075.2668157
GCTCAAA76250.074.876268
CTCAAAC76100.074.8346259
GATGGCT77800.073.626354
CTGATGG78250.073.37682
TGGCTCA78950.072.412976
ATGGCTC79800.071.744185
TGATGGC84750.067.781833
TCCGATC851.8007995E-1067.767136
Produced by FastQC (version 0.11.5)