FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005282025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594948
Sequences flagged as poor quality0
Sequence length8
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATATTC58495698.32052549130344No Hit
TGATATTA25870.4348279177339869No Hit
TGATAATC11900.2000174805193059No Hit
AGATATTC10660.17917532288536142RNA PCR Primer, Index 37 (100% over 8bp)
TGAAATTC10350.17396478347687527No Hit
TGATATAC8540.14354195660797245No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)