FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594948
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2600.0127.36291
TATTGAG900.0119.9795463
GTTATGC850.0118.5680168
TAGTTGG2750.0117.7980961
TGCGTGG1800.0115.980231
CTCCGTA501.8189894E-12115.180367
CGTTAGA2500.0112.3008651
GCGCGAT1550.0111.464871
GACCAAT1950.0110.750351
TACGGAT2350.0110.279071
GTCGTTA600.0107.98165
TCCGTAG1600.0107.981598
TTACGGA405.0549716E-9107.981599
ACTTTAG1000.0107.981583
TATTACC1950.0107.058671
CTGCGTA1150.0106.416649
TTGAACG950.0106.087181
TCGCCAG750.0105.582018
GGATTAC2050.0105.347891
GTCGTAA2000.0104.38221
Produced by FastQC (version 0.11.5)