FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594948
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC209783.526022442297478No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC159282.677208764463449No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC151252.542238985592018No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC116721.9618521282532255No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA93681.574591392861225No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG46080.7745214707840012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30050.505086158790348No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA11730.19716008794045864No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG9660.1623671312450836No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA8240.1384994991158891No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG8020.134801696955028No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT7460.12538910963647243No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA6940.11664884998352798No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG6890.11580844040151407No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA6690.11244680207345853No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA6620.11127022865863909No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC6500.10925324566180573No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG6430.10807667224698629No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG100.007236724144.010936
TCTGATG41050.0126.837341
GTCTCTC55850.0122.077382
AGTCTCT55900.0121.911051
TCAGCTG56050.0121.1235357
AGCTGGT57700.0118.283739
TCTCTCA58550.0116.748373
CAGCTGG59700.0114.9240658
CTCAGCT60350.0113.1088266
CTCTCAG61750.0110.5444264
TCTCAGC63850.0106.795885
GGCTCAA71350.072.851027
CTCAAAC72150.072.442389
GCTCAAA72200.072.192798
GATGGCT73350.070.974724
CTGATGG73350.070.942152
TGGCTCA76350.068.185926
ATGGCTC76750.068.1120155
TGATGGC79900.065.268453
TCAAACA74250.058.661269
Produced by FastQC (version 0.11.5)