FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689519
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT2700.0133.42291
CGATTGA2000.0118.87981
GTACGTA2450.0114.689231
TACACGA1900.0113.7605741
CTCTACG2050.0112.4657441
GGTATTG2650.0111.4710541
TACCTAT3250.0110.843641
TATTGCA1650.0109.1325153
TAGTTGG3050.0108.66311
CGCTATC2800.0108.0725561
CCTTCGA1600.0108.072551
CTTGACG1800.0107.994189
TTGAACG1550.0106.910481
TACCTTA2050.0105.436631
CTGCGTA1300.0105.225099
CCGGTAG2000.0104.470121
CATCGAC900.0103.99448
TCCGTAG2300.0103.2987758
CTACCCA3150.0102.926231
TTTATGC2450.0102.926231
Produced by FastQC (version 0.11.5)