FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689519
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC246193.5704599873244973No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC192652.7939766706936284No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC182352.6445971757123443No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC140522.037942391725246No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA115621.6768210883238894No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG63830.9257177829762486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35490.5147066288238612No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA14120.20478043389667291No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG11040.16011161403819185No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10150.14720406544272166No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA10000.14502863590415926No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT9350.1356017745703889No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG8820.1279152568674685No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA8460.12269422597491875No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG8190.11877845280550645No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8000.11602290872332742No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7910.11471765100018999No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA7800.11312233600524423No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA7770.11268725009753175No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT7290.1057258755741321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG53600.0129.881131
GTCTCTC71300.0122.596672
AGTCTCT72050.0121.579581
TCAGCTG72400.0120.921657
TCTCTCA74600.0117.659543
AGCTGGT74400.0117.2849
CAGCTGG75100.0116.2866748
CTCAGCT75950.0115.459216
CTCTCAG76750.0114.399314
TCTCAGC80450.0109.008865
TTCCGAT502.055458E-10100.801885
TGATACG353.5408644E-582.3589253
TACGGCG353.5574685E-582.281286
CCGATCT651.6516424E-977.534287
GGCTCAA92350.076.089037
CTCAAAC93350.075.4281859
GCTCAAA93050.075.361888
GATGGCT94300.074.318894
CTGATGG94100.074.114372
TGGCTCA96850.072.479326
Produced by FastQC (version 0.11.5)