FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005282031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673544
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATCGA66364298.52986590334113No Hit
GTGATCAA13480.200135403180787No Hit
GTGATCGC12970.1925635147815139No Hit
GTGAACGA11560.17162947038352358No Hit
GAGATCGA9530.1414903851864169RNA PCR Primer, Index 21 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)