FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673544
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC243473.614760134452983No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC188642.800707897331132No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC176452.6197249177485062No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC139292.0680163434014704No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA113751.6888280498378725No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG59780.8875440951147957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33600.4988538239521101No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA13620.20221396078058748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG10700.15886118798474932No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10110.15010155238559025No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA9490.14089651158647393No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT9330.13852101718670198No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9090.13495777558704405No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA8870.1316914707873576No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8690.12901903958761415No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG8030.11922012518855486No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8010.11892318838858337No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7640.11342985758911073No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA7200.10689724798973786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG47650.0127.790531
GTCTCTC72600.0122.2139052
AGTCTCT73000.0121.806141
TCAGCTG73850.0120.498327
AGCTGGT75050.0118.283839
TCTCTCA76450.0116.221283
CAGCTGG77350.0115.045918
CTCAGCT78000.0114.087196
CTCTCAG79150.0112.4629754
TCTCAGC83750.0106.3482065
GGCTCAA84800.073.1004947
CTCAAAC85700.072.4168249
GCTCAAA86400.071.830128
GATGGCT86400.071.434684
CTGATGG86350.071.0082242
ATGGCTC88350.070.0054555
TGGCTCA88150.069.914056
TGATGGC95200.064.264463
TCAAACA88600.058.3450139
AGTCTAT605.034779E-448.1551481
Produced by FastQC (version 0.11.5)