FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708788
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATCGA69930998.66264665880348No Hit
GTGATCAA14690.2072552018375029No Hit
GTGATCGC12140.171278294779257No Hit
GTGAACGA11720.16535268655789884No Hit
GAGATCGA8870.12514320219868283RNA PCR Primer, Index 21 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)