FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708788
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA2050.0119.502651
CGATTGA3200.0119.33791
CTAAGCG1350.0117.4032445
AATCCGA1550.0116.091659
GGTATTG2600.0113.6221541
TACACGA2250.0112.0825651
GGCTCGA1800.0112.082551
TACCTTA2450.0108.814851
TCCGTAG2400.0107.965248
CCGGTAG1950.0107.155851
GTACGTA2400.0105.077391
CCGTACT2550.0104.547591
GTAGCGT2350.0104.136688
TTAGGCA2150.0103.890471
GCATGCG2100.0102.932961
TAGTTGG3300.0102.621051
TATTGCA2250.0102.461013
GTATTGC1550.0102.2688752
CTACCCA2700.0101.408031
TGTTCCG2150.0100.539171
Produced by FastQC (version 0.11.5)