FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684594
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTGG2300.0122.082561
CGATTGA2150.0120.55381
TTGAACG1950.0118.149591
TAAACCG2900.0114.202791
GAGCGAC950.0113.663512
GCGCGAT2050.0112.38621
CGTTGGG1950.0110.765241
GCATGCG2300.0109.561271
GGTATTG2950.0107.385961
ACTTCGA950.0106.0859459
TACGGAT2250.0105.596211
ACGAGGA2600.0105.2269741
CTTTGCG2700.0103.9962541
AACGTTG2500.0103.676271
AGCGTAT2300.0103.300631
CATCGAA2800.0102.853441
GTCGAAA1050.0102.838418
CTACCCA2950.0102.5047761
TACGGTA2600.0102.457851
ACTTTAG1700.0101.628553
Produced by FastQC (version 0.11.5)