FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684594
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC237873.474614150868982No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC183492.6802747321770277No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC174252.545304224109472No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC135421.9781067318731977No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA109551.6002185236797286No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG54720.7993058659585097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33120.4837903925538348No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA12990.1897474999780892No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG10610.15498236911220373No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG9940.14519554655752168No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA9340.13643122785183628No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT8880.12971191684414413No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG8470.12372296572859243No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8100.11831830252675309No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG7680.11218327943277329No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7540.11013827173478002No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7460.10896969590735531No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA7330.10707076018779015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG47250.0128.756881
GTCTCTC63450.0122.0720142
AGTCTCT65550.0118.213081
TCAGCTG66000.0117.815317
AGCTGGT66950.0115.9200069
TCTCTCA67000.0115.793693
CTCTCAG69450.0111.875394
CTCAGCT70000.0111.391576
CAGCTGG69900.0111.336798
TCTCAGC73200.0106.635935
GGCTCAA82700.073.913567
CTCAAAC84400.072.6753859
GCTCAAA84350.072.633118
TTCCGAT801.05501385E-1072.0087665
CTGATGG85800.071.29512
GATGGCT85700.071.252554
TGGCTCA87550.069.736746
ATGGCTC88050.069.269085
CTTCCGA851.8007995E-1067.772964
TGATGGC92900.065.909383
Produced by FastQC (version 0.11.5)