FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773922
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2900.0124.225221
GATTGAT1050.0123.49942
TTGACTT3150.0114.366071
TAGTTGG2850.0113.7641451
GGTATTG2750.0112.660991
CTTTGCG2950.0112.3501361
GTACGTA2850.0111.236051
TACACGA3100.0109.2380451
CACCGAA2000.0107.992158
GTACGAT1000.0107.992159
TACGGAT3500.0107.046641
ACCGCGA1500.0105.660592
GTAATTG750.0105.592338
TACGAAA553.6379788E-12104.7196669
CCGGTAG2150.0103.886951
GTCGTAA2500.0103.75291
CTTGACG1750.0102.849669
GTCCGAA2050.0101.846258
CGCTATC3400.0101.718531
CTCTACG3050.0101.579571
Produced by FastQC (version 0.11.5)