FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746378
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC261073.497825498607944No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC200052.6802772857720885No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC187092.506638727293677No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC146271.9597308602343586No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA121781.6316129360726066No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG64530.8645753224237585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58650.7857948653363309No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA14670.19654920161097997No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG11670.15635509085208835No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10090.1351861925190721No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA9990.13384638882710906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9750.13063085996639773No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT9700.1299609581204162No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA9310.1247357237217603No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG9040.12111825375346005No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8900.11924252858471177No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8570.1148211764012337No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7970.10678235424945537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG53300.0129.910981
GTCTCTC73400.0123.1157762
AGTCTCT75150.0120.674391
TCAGCTG76450.0118.751527
AGCTGGT77700.0117.026419
TCTCTCA77800.0116.607393
CAGCTGG79200.0114.810018
CTCAGCT79450.0114.086286
CTCTCAG81100.0111.5511554
TCTCAGC85050.0106.743725
CTTCCGA352.8704744E-7102.898074
GGCTCAA94250.074.095527
CTCAAAC94850.073.930439
GCTCAAA95600.073.199818
TTCCGAT502.4034507E-671.994875
GATGGCT97050.071.843114
CTGATGG97950.071.126492
ATGGCTC99450.070.2212455
TGGCTCA99700.069.972956
TGATGGC105000.066.430273
Produced by FastQC (version 0.11.5)