FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757611
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA650.0132.922419
CGATTGA2650.0116.9144741
TACACGA2300.0115.9096451
GGCTCGA2150.0113.9426041
ACGATAC2350.0113.443491
GGTATTG3050.0113.393231
TCCGTAG2400.0110.999448
GTATTGC1500.0110.3994452
GTCGTAA1450.0109.320191
TAGACTA800.0107.999467
AGCGTAT2100.0106.362391
TAAACCG3350.0105.389411
GACCAAT2200.0104.802831
TTCCGTA1100.0104.726757
TACGGTA3100.0104.591531
CGTTAGA2550.0104.545961
TTGGACG1450.0104.275342
TAGTTGG3150.0102.931351
ATAGTTA1050.0102.856626
GATTACG850.0101.6465452
Produced by FastQC (version 0.11.5)