FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757611
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC273693.6125399446417754No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC206402.724353263086201No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC196952.5996190657210625No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC152232.009342525385719No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA124181.6390997490796728No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG64340.8492484929601075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38480.5079123719164584No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA14290.18861922543363283No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG11890.156940699118677No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA10800.1425533684173012No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10740.14176140525942732No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT10690.1411014359611991No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9850.13001395175096456No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA9210.12156634473364299No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA9170.1210383692950604No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG8880.11721054736533655No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC8610.11364671315490403No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8590.11338272543561273No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA7660.10110729648856735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG55750.0130.993521
GTCTCTC74900.0122.511612
AGTCTCT74950.0122.494731
TCTCTCA78400.0117.523613
TCAGCTG78600.0116.878917
AGCTGGT79350.0115.139079
CAGCTGG79900.0114.526718
CTCAGCT82950.0111.190946
CTCTCAG83100.0110.68754
TCTCAGC86600.0106.345235
CGATCTA406.375376E-789.994938
CTTCCGA801.8189894E-1281.032874
TTCCGAT801.8189894E-1281.006135
GGCTCAA93650.078.5689857
CTCAAAC93700.078.5270549
GCTCAAA94150.078.0752648
GATGGCT96250.076.40714
CTGATGG96450.075.9013062
TGGCTCA98650.074.372746
ATGGCTC100950.072.754395
Produced by FastQC (version 0.11.5)