FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768699
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2000.0129.598831
TAGTTGG4050.0117.332271
ATAGTTA750.0115.191476
TACGGAT3000.0112.798991
CGTTAGA2150.0107.161821
TGACTAG1950.0107.075951
TCGAATG1750.0106.956548
CCGGTAG2300.0106.4338151
GTCGAAA1150.0106.4199758
CGCCGAA1500.0105.592182
GATTTCG1100.0104.719512
GATTGAC553.6379788E-12104.719512
GTCGTAA2150.0103.813011
TACACGA2850.0103.578011
GTTATGC1600.0103.48568
ATACTAC1050.0102.8428349
GGCTCGA2250.0102.399081
CTCTACG3250.0101.906771
ACTTTAG2050.0101.846113
TACGGTA3400.0101.646131
Produced by FastQC (version 0.11.5)