FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768699
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC267833.4841986265105067No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC202072.6287272391404177No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC190702.48081498740079No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC150041.9518693272659389No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA117701.5311584898640431No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG56150.7304549635162788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36330.47261671993849347No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA13950.1814754539813373No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG12120.1576689965773339No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10770.14010685586946256No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT10350.13464307876034703No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA10080.13113065061877274No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9010.11721102798364509No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8880.11551985887844267No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG8740.11369859984207081No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA8540.11109680121868248No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC8120.10563302410956696No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8040.1045923046602116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG53450.0130.279391
GTCTCTC73750.0121.46252
AGTCTCT74600.0120.1098251
TCAGCTG76900.0117.5045857
TCTCTCA76650.0117.49463
AGCTGGT78450.0115.07629
CTCAGCT80250.0112.606736
CAGCTGG80400.0112.561118
CTCTCAG81950.0109.9336244
TCTCAGC86450.0104.377825
TTCCGAT608.749339E-1084.017355
TGATACG353.5451176E-582.340213
ATACGGC353.5531382E-582.3027045
GGCTCAA93400.074.8530967
CTCAAAC94550.074.237619
GCTCAAA94750.074.009748
CTGATGG96200.072.441022
GATGGCT96400.072.313684
GATACGG406.8875204E-572.014874
TGGCTCA99400.070.1220556
Produced by FastQC (version 0.11.5)