FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728080
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG1350.0128.103681
CGATTGA3050.0125.2160951
TACGGAT2550.0121.510111
GGCTCGA1700.0118.6842961
TAGTTGG3000.0115.293311
CTCTACG2500.0115.293311
GACCGGA1250.0115.19839
GATTACG1000.0115.19832
GGTATTG2250.0112.090711
TCGAATG1600.0107.9984058
CGAACAT3150.0107.515591
GCATGCG3100.0106.925251
TAAACCG2500.0103.763981
ACGATAC2650.0103.328911
CCTTCGA1050.0102.940461
TTGAACG1400.0102.940451
GTCGAAA1050.0102.855628
TATTACC2250.0102.482941
GTGTGAC2050.0101.852168
CATCGAA2550.0101.729391
Produced by FastQC (version 0.11.5)