FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728080
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC257293.533814965388419No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC196582.699978024392924No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC184052.5278815514778596No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC144911.9903032633776507No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA115221.5825184045709262No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG60400.8295791671244918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55270.7591198769366003No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA13940.19146247665091748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG11170.1534172069003406No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG10580.14531370179101197No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9930.13638611141632787No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA9840.13514998351829471No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT9690.13308977035490605No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG8780.12059114383034832No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA8530.11715745522470059No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8080.11097681573453468No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8030.11029007801340512No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC7990.10974068783650148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG51600.0129.760031
GTCTCTC71500.0123.5031052
AGTCTCT72700.0121.3087541
AGCTGGT74550.0117.9165659
TCAGCTG75050.0117.514717
TCTCTCA75500.0117.078493
CAGCTGG76600.0115.418768
CTCAGCT77850.0113.103146
CTCTCAG79650.0111.150554
TCTCAGC81400.0108.465645
ATACGGC259.639054E-486.4186255
TACGGCG259.6495805E-486.394896
GGCTCAA90100.074.392947
CTCAAAC91050.074.170249
CTGATGG90550.074.096382
GCTCAAA91600.073.4891058
GATGGCT92850.072.617194
AGTCTAT608.239658E-872.228881
TGGCTCA95150.070.520276
ATGGCTC95550.070.319715
Produced by FastQC (version 0.11.5)