FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766852
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC276333.603433257003959No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC213142.7794150631412577No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC198032.5823757387344624No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC153432.0007772034238682No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAA124401.6222165424358286No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG66200.8632695748332142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56410.7356047842347676No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGTAAAA15420.2010818254369813No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG11910.15531028151455561No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG11310.14748608597226062No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA10520.1371842285082389No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT10440.13614100243593288No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG9560.1246655156405669No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA9360.12205745045980189No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG9330.12166624068268714No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA8980.1171021266163484No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC8870.11566769076692765No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA8460.11032115714635941No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA8050.10497462352579115No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT7790.10158413879079665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG54400.0132.3261
AGTCTCT81950.0122.871181
GTCTCTC82800.0121.546032
TCAGCTG83400.0120.1769567
AGCTGGT85850.0116.92279
TCTCTCA86600.0116.4621663
CAGCTGG86700.0115.9349448
CTCAGCT87750.0114.301526
CTCTCAG90450.0111.649684
TCTCAGC92100.0109.2227555
GATACGG301.6516218E-596.0410164
TGATACG353.546946E-582.331623
CTTCCGA353.549243E-582.320884
TTCCGAT353.5549907E-582.294035
CGCTCAA353.556141E-582.288667
GGCTCAA96150.075.3349847
CTCAAAC96750.074.947119
GCTCAAA97600.074.5108648
CTGATGG98050.073.7667852
GATGGCT98200.073.497794
Produced by FastQC (version 0.11.5)