FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987861
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC349923.542198750633945No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC269842.7315583872629854No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC207492.100396715732274No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC162191.6418301765126875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45120.4567444205207008No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA27440.2777718727634758No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA24830.2513511516296321No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG15360.15548746230491942No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA15100.15285551307319553No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG14630.14809775869277156No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA14610.14789530105956203No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG14330.1450608941946286No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT12110.1225880969083707No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGC11960.12106966465929923No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG11960.12106966465929923No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC11830.11975369004343728No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC10890.11023818128258936No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT10290.10416445228630343No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG10070.1019374183209986No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCC9990.10112758778816047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG67750.0129.176241
GTCTCTC91700.0121.592342
TCAGCTG94450.0119.063987
AGCTGGT94650.0118.1278159
AGTCTCT96100.0116.5737841
TCTCTCA97300.0114.923823
CTCAGCT98600.0113.839386
CAGCTGG99950.0112.29618
CTCTCAG100600.0111.400954
TCTCAGC106250.0105.601915
GGCTCAA114700.076.827977
CTTCCGA851.8189894E-1276.260714
CTCAAAC115500.076.108839
GCTCAAA116550.075.36148
GATGGCT118150.074.4319154
TGGCTCA119500.073.625236
CTGATGG119550.073.37132
ATGGCTC125150.070.7219545
TGATGGC140450.062.482263
TCCGATC1201.8189894E-1259.9987346
Produced by FastQC (version 0.11.5)