FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944810
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT2050.0126.565791
TACGGAT2650.0114.22761
CTAATGG3450.0112.8086241
TACACGA3200.0112.612781
CCTTACG2700.0112.1122741
AGCGATA1800.0111.963958
GTCGTAA3650.0110.576491
TTTATGC3400.0108.108271
TGACTAG3700.0107.1343151
TCGACCA1750.0106.936988
GACCAAT4000.0106.3064651
TACCTTA2950.0105.054361
CGAACAT3150.0102.960261
CGATTGA2950.0102.611241
GTATTAG1200.0101.9671558
TATTAGC1200.0101.9671559
GGTATTG3350.0101.1161961
CGTCTAC3000.0100.901051
GTGCATA4150.0100.727391
ATCTAAC2650.0100.495937
Produced by FastQC (version 0.11.5)