FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914955
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2750.0117.837961
TAGTTGG2950.0114.731111
CTAATGG2700.0114.6859051
TAGTGAG1300.0110.751492
CCTTACG2800.0110.589981
ACTTCGA1700.0110.0949
TACCTTA4050.0108.462641
GTCGTAA3400.0108.018121
CGTCTAC2550.0107.312121
ATTGGTG3250.0106.356311
TGACTAG2700.0104.017451
TACGGAT1950.0103.401961
TTGAACG2250.0102.417181
TGAGCCG2250.0102.417181
CCTTCGA1700.0101.6641161
ATTGAAG1350.0101.317113
CGCTATC3450.0100.190721
TACCTAT5400.0100.016781
CGGACGT2450.099.9351351
TCCACGA2450.099.896918
Produced by FastQC (version 0.11.5)