FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993944
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA3550.0123.788331
GCGCGAT1900.0113.748561
TAGTTGG3550.0113.641751
CGATTGA2900.0111.787381
GGTATTG3550.0111.612431
AGCGATA2500.0109.430328
CCTTACG3100.0109.2230761
CCGTTCA3450.0108.583161
GGCTCGA2900.0106.8190461
TTCGAAG3500.0104.973661
ACGTTGA1050.0102.915352
CTAATGG2600.0102.519521
TGTTCCG3800.0102.3736951
TTTATGC3600.0102.057731
TTTGCGA1200.0102.057732
AATAGTC1700.0101.699473
TGACTAG3300.0100.420441
TAAACCG3600.0100.0565951
CGTCTAC3100.099.927491
TACACGA4400.099.874671
Produced by FastQC (version 0.11.5)