FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403264
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC511663.6462133996168933No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC389322.7743888534160357No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC301262.146851910973274No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC233991.6674695566906867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75280.5364635592447322No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA39510.28155785368968345No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA34850.24834956216364135No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG23020.16404610964152147No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA21830.15556588068959226No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA20760.1479408008756727No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG20130.14345126790112198No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG20090.14316621818845207No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT18350.13076655568731188No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC17820.12698964699443582No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG17610.12549313600291892No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGC16890.12036224117486091No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC16040.11430493478062574No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT15440.1100291890905774No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC14640.10432819483717962No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG14400.10261789656116026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG96800.0129.01541
GTCTCTC130650.0124.387772
AGCTGGT133950.0120.8285149
TCAGCTG135250.0120.305927
TCTCTCA137600.0118.249523
AGTCTCT137450.0118.227961
CTCAGCT141100.0115.424216
CTCTCAG142150.0114.444144
CAGCTGG145450.0112.017718
TCTCAGC152050.0107.0323945
GGCTCAA165050.076.032237
CTCAAAC166100.075.724979
GCTCAAA166600.075.5409248
GATGGCT169700.073.839984
CTGATGG172050.072.642842
TGGCTCA173900.072.041216
CTTCCGA1400.072.01524
CGATCTA502.4040874E-671.997248
ATGGCTC180700.069.539345
TTCCGAT1500.067.20945
Produced by FastQC (version 0.11.5)