FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005282089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250848
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCGT123434998.68097482667758Illumina PCR Primer Index 6 (100% over 8bp)
ATTGGCGA34210.2734944613574151No Hit
AATGGCGT20600.16468827547391848No Hit
ATAGGCGT13410.10720727058763337No Hit
ATTGGCGG13100.10472895187904525No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)