FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250848
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT3050.0115.712871
CGATTGA3650.0110.504661
TGACTAG4150.0109.339691
TAGTTGG4100.0108.91641
ACGAGGA4050.0108.482631
TGTTCCG3700.0107.064711
CCTTACG3600.0106.037321
TAAACCG4400.0104.764151
TACCTTA4800.0102.035921
GCATGCG3900.0101.574211
TACCTAT5900.0101.32381
GTTTGAC2950.0100.063028
CGTCTAC3800.098.561011
GCGCGAT3450.098.121491
CGTTGGG3450.098.121491
TTTATGC5000.097.954481
CCGTTCA5100.097.446071
CCTTCGA3850.097.2811
CTAATGG4450.097.112841
CATATTC3000.096.0338061
Produced by FastQC (version 0.11.5)