FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285128
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA5400.0109.419241
TTTATGC4500.0108.885491
GGCTCGA3050.0108.675491
TATTGTC3050.0108.671263
TACCTAT6650.0108.355741
AGACGTA2000.0107.967129
GTCGTAA4700.0107.3183061
CGTTAGA3100.0106.922651
CGTCTAC4250.0106.813271
TAGTTGG4350.0106.0142751
GTATTAC3000.0105.567848
GTCTAGT2950.0104.91728
CTAATGG4750.0104.671651
TGACTAG3750.0103.7614751
CTGATAG4950.0101.8981861
CGATTGA3000.0100.87921
GTGCATA5400.0100.0785751
AGCGATA2600.099.661958
TACGGTA3350.098.943351
GGGTCGA2650.097.781539
Produced by FastQC (version 0.11.5)