FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242237
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC460133.70404359232578No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC347612.798258303367232No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC265872.1402518199023213No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC208551.678826182121447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64510.5193050923455025No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA35560.2862577752876464No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA30800.2479398053672528No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG19920.1603558741206388No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA18900.15214488056626874No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA18630.14997138227246493No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG18430.14836138353631392No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG17370.13982839023471366No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT16280.13105389712269078No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC15840.1275118999031586No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGC14920.120105905716864No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG14760.11881790672794322No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC13600.10947991405826747No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT13590.10939941412145991No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC13550.10907741437422973No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG12760.1027179193664333No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA12550.10102742069347476No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC12520.1007859208830521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG81600.0128.602311
GTCTCTC119200.0121.2120062
AGTCTCT121600.0118.89811
TCAGCTG121500.0118.693647
AGCTGGT121950.0118.3147059
CTCAGCT125550.0115.218186
TCTCTCA126950.0114.086933
CAGCTGG127750.0113.224878
CTCTCAG129650.0112.212574
TACGGCG458.913048E-11112.0065166
TCTCAGC138150.0105.1478045
CTTCCGA1200.096.028784
ATACGGC651.6498234E-977.558585
TTCCGAT1550.074.3418665
GGCTCAA145450.073.0131457
CTCAAAC146200.072.9340749
GCTCAAA147250.072.3162168
GATGGCT148950.070.885294
CTGATGG149950.070.356162
TGGCTCA152250.069.332116
Produced by FastQC (version 0.11.5)