FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968983
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC366153.7787040639515865No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC281182.901805294829734No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC212852.1966329646650147No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC165281.7057058792569118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47320.48834706078434814No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA26670.2752370268621844No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA23140.23880707917476365No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG16940.17482246850563943No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA15360.15851671288350774No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG14590.15057023704234232No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA14450.14912542325303954No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG14050.14499738385503153No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT13060.13478048634496168No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGC12740.13147805482655528No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC12210.12600840262419463No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG11490.11857793170778022No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT11410.11775232382817861No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC10810.11156026473116659No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC10630.10970264700206299No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG10370.1070194213933578No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC10090.10412979381475218No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCC9850.10165297017594736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG68250.0129.718861
GTCTCTC100100.0122.512612
TCAGCTG100900.0121.588337
AGCTGGT101950.0120.618569
AGTCTCT102500.0120.1905751
CTCAGCT105950.0115.996816
TCTCTCA106300.0115.5368963
CAGCTGG107300.0114.537398
CTCTCAG108050.0113.6991964
TCTCAGC113450.0108.524435
TTCCGAT502.055458E-10100.80085
CGATCTA406.376249E-789.996068
GGCTCAA117250.075.957447
CTCAAAC117650.075.760399
GCTCAAA118400.075.34138
GATGGCT120100.073.8704764
CTGATGG121450.073.118362
TGGCTCA123050.072.3771746
ATGGCTC125000.071.5397645
CTTCCGA755.120455E-967.210944
Produced by FastQC (version 0.11.5)