FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313554
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4550.0118.689861
CGTTAGA4650.0116.137381
TACGGAT4850.0114.3175051
ATTACGA1450.0114.184679
TAGTTGG5150.0109.056381
CCTTACG3700.0108.980811
TGACTAG3200.0108.007771
GGTATTG3900.0107.0846251
GTTAACG2050.0105.345369
TAAGCGT4250.0105.042851
CTTTGCG2950.0104.9566961
GGCTCGA3700.0103.142551
TACCTAT4700.0102.645681
TGACGGC3750.0101.767321
GGATTAC5750.0101.433381
GCGCGAT3350.0101.0221941
AGCGATA3600.099.9805458
GGAATAA6950.099.460391
GCCTCTA4350.099.317481
GTTTGAC3200.098.980748
Produced by FastQC (version 0.11.5)