FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465825
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTGG5500.0123.114861
CGTTAGA4400.0117.875921
TCGAATG2300.0112.6893548
CTAATGG4900.0111.72821
TGACTAG4900.0111.72821
CGATTGA4600.0111.18491
CCTTACG4300.0108.8905641
GGATTAC5400.0108.052931
TTGAACG5750.0107.739741
GGTATTG4950.0106.2338641
TACGGAT5350.0105.0234151
GGCTCGA4000.0104.4511641
CCGGTAG3000.0100.84941
CACCGAA3550.099.3747258
GCGCGAT3800.098.574611
TCGTTTA6450.097.110858
GCAAACT7800.096.047051
CCGTACT5050.095.571571
TATTGTC3550.095.354393
TATTGAG3250.095.291973
Produced by FastQC (version 0.11.5)