FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428343
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA4350.0120.925921
TAGTTGG5750.0120.306531
CTAATGG5550.0112.956721
CGATTGA4900.0111.764351
GGAATAA6850.0111.506741
TGACTAG5500.0111.363291
CCGGTAG2850.0111.248361
TCGAATG2750.0109.960688
ACGCCGA5650.0109.682111
CGTGGGA4000.0107.9970869
GTCCGAA4100.0105.3630148
TTGAACG5450.0103.129741
TACGGAT5250.0102.940861
TAAACCG4700.0102.721831
GCGCGAT3700.099.324011
TACCTAT4800.097.5793461
TGGTTAG5700.097.3423161
AACGTTG5800.096.9063951
GTAAACG2750.096.870128
CTCTACG4200.096.0781251
Produced by FastQC (version 0.11.5)