FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507675
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG4450.0121.43661
CTAATGG4500.0116.884991
TAGTTGG5100.0114.436141
ACTTTAG3100.0113.8779143
CGATTGA4400.0112.991241
TACGGAT5450.0111.053221
CGTTAGA4700.0110.378111
CCGGTAG2950.0109.9104161
AGCGAAC3300.0109.065289
GGTATTG4550.0107.682681
CCTTACG4250.0106.807061
TACACGA4650.0105.366931
TACCTTA3900.0105.307331
TAAGCGT4750.0104.665571
GGCTCGA4100.0103.6851351
TTGAACG5450.0103.120851
ACGAGCC4400.0101.528361
AACGTTG6050.0101.230631
GGAATAA8400.0101.216441
TAAACCG4700.0101.179941
Produced by FastQC (version 0.11.5)