FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456601
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC572053.92729374756711No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC372812.5594517647591895No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC319812.195590968288502No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC216941.489357758233037No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60510.41541918480077933No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA46770.32108998964026525No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA37340.25635022906066934No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG25070.17211302202868184No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA24810.1703280445365615No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA24020.16490445907973425No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG23620.16215833986108757No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT17770.12199634628837959No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG17290.11870100322600355No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA16590.11389529459337182No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG16420.11272819392544697No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT16370.11238492902311614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG108100.0130.333791
GTCTCTC135450.0122.929682
TCAGCTG136400.0122.29637
AGCTGGT136300.0122.162169
AGTCTCT140050.0119.2891851
CTCAGCT141600.0117.994186
TCTCTCA143050.0116.820013
CAGCTGG144400.0115.409288
CTCTCAG148050.0113.180444
TCTCAGC157750.0106.122395
CTCAAAC172550.082.271399
GGCTCAA171650.082.2499167
GCTCAAA173650.081.4185948
GATGGCT175650.080.180494
CTGATGG177300.079.440072
TGGCTCA180200.078.211726
ATGGCTC188850.074.876165
TCCGATC1850.073.963556
CTTCCGA1950.070.192244
TTCCGAT1950.070.1874165
Produced by FastQC (version 0.11.5)