FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491998
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG4950.0119.3387151
TAGTTGG5900.0117.217361
CGTTAGA4750.0115.263731
TACGGAT5850.0114.524871
CTAATGG4850.0114.372521
TATTGAG3050.0113.351363
CCTTACG4550.0110.830511
TACACGA5500.0104.785221
CTAATCG1650.0104.764144
CGCTATC4900.0104.384251
CGATTGA4650.0103.799331
CTCTACG4150.0102.4180761
GGAATAA7750.0101.320551
CGTGGGA2850.0101.050949
GGTATTG4950.0100.419171
TACCACG3150.098.320315
TTGAACG4800.097.553941
GTATTGA1700.097.446052
TACGGTA4450.097.132371
GGATTAC6450.096.0531161
Produced by FastQC (version 0.11.5)