FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453569
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA4700.0121.106621
TAGTTGG5850.0117.005531
CTAATGG4950.0113.534551
TGACTAG4450.0110.100061
CGATTGA3900.0108.999891
GGTATTG4350.0107.662071
AATCCGA4300.0107.160899
CGTGGGA3400.0103.762879
CGGCTAG4550.0102.929681
TCGTTTA6350.0100.9116068
CCTTACG4400.098.251051
TAAGCGT4700.098.1116941
CTCTACG4850.098.048481
TTTATGC5600.097.7831951
TACGGAT5500.096.941051
TACACGA4850.096.56291
AACGTTG5300.096.520851
GGAATAA6900.096.06771
CCGGTAG3450.096.06771
CTCGTAC6000.096.0676961
Produced by FastQC (version 0.11.5)