FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319232
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA3550.0115.665551
TAGTTGG4700.0113.420451
CGATTGA3950.0113.071221
CTAATGG5300.0111.453961
TACGGAT4850.0109.9126051
GGTATTG5200.0109.4413151
ATACTAC2700.0103.994749
TGACTAG4300.0103.867771
GTCCGAA3700.0103.129928
GATCGTA5850.0102.2067951
AGCGATA3100.0102.188388
ATTACGT2750.0102.1465153
GTCGACT3300.0100.3585748
TTGAACG4400.099.869921
CTTTGCG3400.099.580991
ACTTTAG2250.099.236363
ACGCCGA5600.099.0513151
CCGGTAG2550.098.874741
CCTTACG3150.098.3366551
GTACGAA1400.097.709358
Produced by FastQC (version 0.11.5)