FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488665
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT5750.0120.215041
TGACTAG5000.0119.5263061
CGTTAGA4100.0115.908561
GGTATTG5000.0113.7661
CTAATGG5300.0111.40211
GGAATAA8450.0109.922961
CCTTACG4300.0108.842951
TAGTTGG6550.0108.830171
TCGTTAC2500.0106.5441368
GGCTCGA4650.0105.2958761
CGATTGA4250.0103.3466341
CGTGGGA2800.0102.8418359
GTCTAGT2550.0101.6319358
ATTACGA2150.0100.450169
TGAGCCG3800.0100.4263461
TCGAATG2800.0100.270798
CGGACGT3950.0100.2584461
CTTTGCG4100.0100.1028441
TAAGCGT5500.099.4961551
TTGAACG5600.099.0052261
Produced by FastQC (version 0.11.5)