FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488665
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC604334.059543282068162No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC381132.5602133455142693No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC339662.2816416050622537No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC221971.491067500075571No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59590.4002915363765521No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA48690.327071570836958No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA35940.2414243634397262No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA25610.1720333318778906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG25170.1690776635441822No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA25050.16827157218044356No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG23570.1583297786943335No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT18210.1223243644473404No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG17300.11621150493898895No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG17030.11439779937057701No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT16430.11036734255188373No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA16190.10875515982440642No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG15310.10284382315698964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG114000.0128.840331
GTCTCTC138800.0123.463832
TCAGCTG141050.0121.533217
AGCTGGT141250.0121.1531759
TCTCTCA145550.0117.908393
AGTCTCT145850.0117.365361
CTCAGCT147500.0116.222626
CTCTCAG150000.0114.345094
CAGCTGG151650.0113.224418
TCTCAGC159050.0108.103195
TTCCGAT1200.084.0010455
GGCTCAA179250.082.137767
CTCAAAC179800.081.8036659
GCTCAAA181100.081.295968
GATGGCT183950.079.73674
CTGATGG187000.078.538012
TGGCTCA188250.078.06056
TCCGATC1300.077.536816
ATGGCTC194850.075.52985
TGATGGC220500.066.557593
Produced by FastQC (version 0.11.5)