FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479498
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG4800.0118.553141
TAGTTGG4900.0117.6037451
CGATTGA4450.0114.9279251
CGTTAGA4900.0114.663651
GGTATTG5600.0108.048441
TACACGA5200.0108.048431
CATCGAA4150.0107.61451
TGACGGC3900.0105.277971
GGATTAC6250.0102.573981
CTAATGG5200.0102.5074841
ACGCCGA5850.0102.199661
TACGGAT5300.0101.932491
CCGGTAG3350.0101.060231
GGCTCGA3800.0100.4660951
TGGTTAG4850.099.508521
ACTTTAG2250.099.217663
TATGAAT2700.098.660879
GGAATAA6950.098.461411
TTCGCGA3000.098.3942269
GCGCGAT4250.098.3028951
Produced by FastQC (version 0.11.5)