FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319232
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC545444.134526754960461No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC348812.6440383495852133No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC302752.2948958181729977No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC198391.5038295008004658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69410.5261394508319992No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA41820.3170026197060108No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA31790.24097353611798378No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA23430.1776033328482026No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG22700.1720698103138796No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA22680.17191820695677484No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG22360.16949255324309903No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT16350.12393574443312473No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA16110.12211650414786786No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT15750.11938764371998253No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG14980.11355091447145006No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG14300.10839640032988891No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC14150.10725937515160336No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC13510.10240806772425168No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA13450.10195325765293747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG103650.0130.785141
GTCTCTC123000.0123.7664952
TCAGCTG124600.0121.467237
AGCTGGT125200.0120.875979
TCTCTCA129100.0118.340363
CTCAGCT128850.0117.740166
AGTCTCT130800.0116.503281
CAGCTGG133200.0113.94918
CTCTCAG134200.0113.6216354
TCTCAGC141800.0107.292115
TCCGATC1500.0105.602916
CCGATCT1750.090.516797
CTTCCGA1800.088.032464
TTCCGAT1850.085.6239855
GGCTCAA168650.081.4158257
CTCAAAC170450.080.93019
GCTCAAA170950.080.615278
GATGGCT172100.079.7691044
CTGATGG173250.078.7531362
TGGCTCA176600.077.8322756
Produced by FastQC (version 0.11.5)