FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444429
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTGG5100.0122.926041
CGATTGA4700.0114.989521
CGTTAGA4950.0113.549241
TGACTAG4400.0111.36561
CGTCTAC3000.0110.492141
CCTTACG4200.0108.090141
CCGGTAG2400.0108.090131
TACGGAT4550.0107.694211
CTAATGG5000.0106.648941
AGCGAAC2750.0104.7239769
GGTATTG4900.0104.4136051
TACACGA4350.0104.362891
CATCGAA4800.0103.586381
TTGAACG5800.0103.1204761
GTCCGAA4400.0103.087668
GTAAACG2700.0101.330158
GGATTAC5000.0100.884131
GGCTCGA3650.0100.68671
TATTGAG3300.0100.360483
TACCTTA3250.099.775521
Produced by FastQC (version 0.11.5)