FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527853
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4500.0116.909461
CGTTAGA4300.0113.967171
GGAATAA7850.0112.002971
TACGGAT5300.0111.500631
TAGTTGG5500.0111.3770141
GGTATTG5600.0105.527381
TTGAACG4700.0104.2678451
CTAATGG5400.0104.097471
ACGCCGA6450.0101.67661
CGTCTAC4850.0101.043061
TGACTAG5100.0100.329241
GTCCGAA4550.099.668188
TGCCCTA7100.099.473421
CAATGGA8250.099.466838
ATACTAC3550.099.3562249
TATTGAG3050.099.2177053
CCTTACG4800.099.092781
CTCTACG3900.097.937851
TACACGA5300.097.902991
AACGTTG5450.097.853091
Produced by FastQC (version 0.11.5)