FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527853
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC643384.211007210772241No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC414272.7114519525111382No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC362202.370646914330109No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC235791.5432767419378697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65760.4304078991892545No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA50560.33092188842774795No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA37810.24747145176924742No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA27260.1784203061420176No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA26670.17455867809272227No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG26570.17390416486402815No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG26040.1704352447519493No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT19650.12861184943839493No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA18960.1240957081604055No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT18020.11794328381068075No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG17730.1160451954474678No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG17590.11512887692729601No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA16430.1075365234744442No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG16290.10662020495427244No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC15600.102104063676283No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC15550.10177680706193594No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA15360.1005332319274171No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG118250.0131.021791
GTCTCTC149300.0124.347872
TCAGCTG150850.0122.341477
AGCTGGT151150.0121.7691659
AGTCTCT155650.0119.5123141
TCTCTCA157350.0118.4704743
CTCAGCT156600.0118.125256
CAGCTGG159800.0115.354248
CTCTCAG164500.0113.488874
TCTCAGC171050.0108.953225
GGCTCAA191350.081.620927
CTCAAAC192000.081.459789
GCTCAAA193850.080.6425868
GATGGCT194750.079.736194
TGGCTCA198950.078.213426
CTGATGG199000.078.1597752
ATGGCTC205300.075.974595
TGATGGC232700.066.891513
TTCCGAT1301.8189894E-1255.3930745
CTTCCGA1353.6379788E-1253.3519634
Produced by FastQC (version 0.11.5)